市售的人5α – 双氢睾酮免疫无效

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所属分类:疗效
摘要

酶免疫测定(EIA)通常用于在生物流体中雄激素的评价;然而,仔细必须考虑到…

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市售的人5α - 双氢睾酮免疫无效

亮点

我们评估了商业双氢睾酮(DHT)免疫的有效性

•[123。 ]用LC-MS / MS相比免疫高估DHT浓度由79-1000%。

免疫分析没有检测到下列非那雄胺治疗。

的交叉反应性在DHT的减少的DHT免疫测定范围为18%至99%,睾酮(T)。

该免疫测定是在含有T.生物流体DHT评估无效

摘要

[ 123]酶免疫测定(EIA)通常用于在生物流体中雄激素的评价;然而,仔细必须考虑到与其他内源性性类固醇激素的交叉反应。我们的目的是要确定一个commonly-的有效性利用市售的二氢睾酮(DHT)EIA。从谁参加了12个月的随机对照(125毫克/周)或车辆试验评估睾酮庚酸的作用,结合非那雄胺(5毫克/天)或安慰剂进行测定老年性腺功能减退的男性获得的血清样品通过EIA和使用经过验证的黄金标准LC-MS / MS方法DHT。另外,市售的(DHT-免费)缓冲液含有分级剂量的睾酮通过DHT免疫测定进行评价。通过EIA测量DHT浓度更高79%至> 1000%,比通过LC-MS / MS(

,P

,P < 0.05), with the largest differences (415–1128%) occuring in groups receiving 非那雄胺. Both LC–MS/MS and EIA indicated that testosterone-enanthate increased serum DHT to a similar magnitude. In contrast, 非那雄胺-induced reductions in DHT were detected by LC–MS/MS, but not EIA ( R = 0.885,< 0.05). No significant associations were present for DHT concentrations between measurement techniques. Cross-reactivity of testosterone with the immunoassay ranged from 18% to 99% and DHT concentrations measured by EIA were highly associated with the spiked testosterone concentrations in DHT-free buffer (获得的值p 图形抽象< 0.001). In conclusion, we provide evidence invalidating a commonly-utilized commercially-available DHT immunoassay because significant cross-reactivity exists between testosterone and the EIA and because the changes in DHT observed via EIA were not associated with a validated gold-standard measurement technique. The cross-reactivity of testosterone is particularly concerning because testsoterone is present in 100-fold greater concentrations than is DHT within the circulation.

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市售的人5α - 双氢睾酮免疫无效关键词

TestosteroneDihydrotestosterone5α-ReductaseFinasteride1

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